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Influence of ellagic acid on oxidative stress tolerance and rpoS gene expression in clinical isolates of Pseudomonas aeruginosa | ||
| زیست شناسی میکروبی | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 مرداد 1403 | ||
| نوع مقاله: پژوهشی- انگلیسی | ||
| شناسه دیجیتال (DOI): 10.22108/bjm.2024.141497.1592 | ||
| نویسندگان | ||
| پارمیس تقی نژاد1؛ لیلا اسدپور* 2 | ||
| 1گروه زیست شناسی، واحدرشت، دانشگاه آزاداسلامی، رشت، ایران | ||
| 2گروه زیست شناسی، دانشگاه آزاد واحد رشت، رشت، ایران | ||
| چکیده | ||
| Due to the emergence of antibiotic-resistant clinical strains of Pseudomonas aeruginosa, there is an urgent need for a suitable and affordable alternative that is a non-antibiotic antimicrobial agent and creates a new generation of infectious disease treatment. In this regard, this study aims to investigate the antimicrobial potential of ellagic acid against clinical strains of P. aeruginosa under physiological growth conditions and oxidative stress. P. aeruginosa isolates isolated from clinical samples were identified by biochemical tests, and their antibiotic sensitivity was checked by the disc diffusion method. The inhibitory effect of ellagic acid against multiple drug resistance isolates was investigated by disc diffusion and broth micro-dilution methods and its effect on bacterial survival in physiological conditions and oxidative stress was investigated by Time-Kill assay. The effect of ellagic acid on rpoS gene expression was also investigated by the Real time-PCR method. In this study, ellagic acid showed an inhibitory effect on the growth of all studied MDR isolates of P. aeruginosa. The minimum inhibitory concentration (MIC) of ellagic acid in 5 isolates varied between 250 and 2000 µg/ml. The ellagic acid treatment made drug-resistant clinical strains of P. aeruginosa sensitive to oxidative stress. It reduced the survival of P. aeruginosa while treatment with 1/4MIC concentration of ellagic acid caused to 4.7 times decrease in log10CFU/mL compared to the control. Also, in the present study, treatment with a sub-inhibitory concentration of ellagic acid caused a significant decrease in rpoS gene expression (P˂0.05). The results of this study indicate the inhibitory effect of ellagic acid on the growth of P. aeruginosa and increase its sensitivity to oxidative stress conditions. Conducting in vivo studies can clarify the possibility of its clinical application in controlling infection caused by resistant isolates of P. aeruginosa. | ||
| کلیدواژه‌ها | ||
| Pseudomonas aeruginosa؛ Ellagic acid؛ oxidative stress؛ rpoS | ||
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آمار تعداد مشاهده مقاله: 53 |
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